Anti-GnRH-R Antibody, clone F1G4

Description

• General description: Gonadotropin-releasing hormone receptor (UniProt P30968; also known as GnRH receptor, GnRH-R, Leutinizing hormone-releasing hormone receptor, Luteinizing-releasing hormone receptor) is encoded by the GNRHR (also known as GNRHR1, GRHR, LHRHR, LRHR) gene (Gene ID 2798) in human. GnRH-R belongs to the family of seven-transmembrane G-protein coupled receptors (GPCRs). GnRH-R resides primarily in the pituitary and is responsible for eliciting the actions of GnRH (also known as Luteinizing hormone-releasing hormone or LHRH) after its release from the hypothalamus. Luteinizing hormone (LH) is then secreted from gonadotropes of the anterior pituitary into the bloodstream following pulsatile stimulation of GnRH-R by GnRH/LHRH. The canonical GnRH-R designated by UniProt (P30968-1) corresponds to a 328-amino acid sequence that includes 4 extracellular domains (a.a. 1-38, 98-115, 185-212, 233-281), 7 transmembrane segments (a.a. 39-58, 78-97, 116-137, 165-184, 213-232, 282-300, 307-326), 4 intracellular domains (a.a. 59-77, 138-164, 233-281, 327-328). Multiple GnRH-R variants and post-translationally processed forms are reported in human brain samples.
• Specificity: Specificity of clone F1G4 was demonstrated by tissue-specific staining and by immunogen peptide blocking (Karande, A.A., et al. (1995). Mol. Cell. Endocrinol. 114(102):51-56).
• Immunogen: BSA-conjugated linear peptide corresponding to the N-terminal sequence of human GnRH-R.
• Application: Detect GnRH-R using this Anti-GnRH-R Antibody, clone F1G4 validated for use in Western Blotting, Dot Blot, ELISA, Flow Cytometry, Immunohistochemistry (Paraffin).
• Quality: Evaluated by Western Blotting in human pituitary tissue lysate.Western Blotting Analysis: 2.0 µg/mL of this antibody detected GnRH-R in 10 µg of human pituitary tissue lysate.
• Target description: ~34 kDa observed. Clone F1G4 was reported to detect multiple GnRH-R variants and posttranslationally processed forms in human brain samples (Wilson, A.C., et al. (2006). J. Endocrinol. 191(3):651–663).
• Physical form: Format: Purified
• Storage and Stability: Stable for 1 year at 2-8°C from date of receipt.
• Other Notes: Concentration: Please refer to lot specific datasheet.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.