Anti-hLYVE-1 Antibody, clone 8C

Description

• General description: Lymphatic vessel endothelial hyaluronic acid receptor 1 (UniProt: Q9Y5Y7; LYVE-1, Cell surface retention sequence-binding protein 1, CRSBP-1,Extracellular link domain-containing protein 1,Hyaluronic acid receptor) is encoded by the LYVE1 (also known as CRSBP1, HAR, XLKD1,UNQ230/PRO263) gene (Gene ID: 10894) in human. LYVE-1 is a disulfide-linked, homodimeric, single-pass type I membrane protein that undergoes ligand-dependent internalization and recycling at the cell surface. It serves as a ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. It is mainly expressed in endothelial cells lining lymphatic vessels. It is expressed on both the luminal and basolateral surfaces of lymphatic endothelium. It is reported to mediate the uptake of intermediate and high molecular weight hyaluronan (HA) and serves as a low affinity receptor for HA-induced lymphoangiogenesis. It also controls the entry and/or migration of leukocytes in lymphatic vessels. The capacity of LYVE-1 to bind HA is based on a receptor distribution and organization of the glycosaminoglycans. (Ref.: Lawrence, W et al. (2016). J Biol Chem. 291(15): 8014 8030).
• Specificity: Clone 8C detects Lymphatic vessel endothelial hyaluronic acid receptor 1. It targets an epitope within the ectodomain region.
• Immunogen: A recombinant fragment corresponding to the ectodomain (1-232) region of human LYVE-1.
• Application: Anti-hLYVE-1, clone 8C, Cat. No. MABS1929, is a mouse monoclonal antibody that detects Lymphatic vessel endothelial hyaluronic acid receptor 1 (LYVE1) and has been tested for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.
• Quality: Evaluated by Western Blotting in lysates from wild-type and LYVE-1 transfected HEK293T cells.Western Blotting Analysis: 2 ug/mL of this antibody detected LYVE1 (Lymphatic vessel endothelial hyaluronic acid receptor 1) in lysate from wild-type and LYVE transfected HEK293T cells.
• Target description: ~60 kDa observed; 35.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Physical form: Format: Purified
• Other Notes: Concentration: Please refer to lot specific datasheet.