Anti-Desmoglein 3 Antibody, clone 5H10

Description

• General description: Desmoglein-3 (UniProt: P32926; also known as 130 kDa pemphigus vulgaris antigen, PVA, Cadherin family member 6) is encoded by the DSG3 (also known as CDHF6) gene (Gene ID: 1830) in human. Desmogleins are protein substances located in and on the surface of keratinocytes. They are present in specialized cell-cell adhesion structures enriched in tissues that undergo substantial mechanical stress. These proteins have been shown to be a critical factor in cell-to-cell adhesion. Desmoglein-3 is a single-pass type I membrane protein that is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It is synthesized with a signal peptide (aa 1-23) and a propeptide (aa 24-49) that are cleaved off in the mature form. It contains an extracellular domain (aa 50-615), a transmembrane domain (aa 616-640), and a cytoplasmic domain (aa 641-999). Desmoglein-3 has four cadherin domains and two desmoglein repeats. This antibody (clone 5H10) recognized the extracellular domain of desmoglein-3.
• Specificity: Clone 5H10 detects Desmoglein-3 in human cells. It targets an epitope with in the extracellular domain.
• Immunogen: Epitope: extracellular domain
• Application: Anti-Desmoglein 3, clone 5H10, Cat. No. MABT1506, is a mouse monoclonal antibody that detects Desmoglein-3 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.
• Quality: Evaluated by Western Blotting in HaCaT cell lysate.Western Blotting Analysis: 1 µg/mL of this antibody detected Desmoglein 3 in HaCaT cell lysate.
• Target description: ~125 kDa observed; 107.53 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Physical form: Format: Purified
• Storage and Stability: Stable for 1 year at 2-8°C from date of receipt.
• Other Notes: Concentration: Please refer to lot specific datasheet.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.