Anti-Podoplanin Antibody, clone 8.1.1

Description

• General description: Podoplanin (UniProt: Q62011; also known as Glycoprotein 38, Gp38, OTS-8, PA2.26 antigen, T1-alpha, T1A, Transmembrane glycoprotein E11) I encoded by the Pdpn (also known as Gp38) gene (Gene ID: 14726) in murine species. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. Podoplanin is detected at high levels in lung and brain, at lower levels in kidney, stomach, liver, spleen and esophagus, and not detected in skin and small intestine. It is expressed in epithelial cells of choroid plexus, ependyma, glomerulus and alveolus, in mesothelial cells and in endothelia of lymphatic vessels. It is also expressed in stromal cells of peripheral lymphoid tissue and thymic epithelial cells and is detected in carcinoma cell lines and cultured fibroblasts. High expression of Podoplanin is observed in colon carcinomas. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. Podoplanin levels are down-regulated by treatment with puromycin aminonucleoside. Mice lacking or with defective podoplanin die at birth of respiratory failure due to a low number of attenuated type I cells, narrow and irregular air spaces, and defective formation of alveolar saccules. Administration of PMab-1 antibody is shown to reduce lymphangiogenesis in the corneal suture and ear wound healing murine models. It suppresses thioglycollate-induced macrophages infiltration at the site of wound healing. (Ref.: Maruyama, Y et al (2014). Invest.Ophthal. Visual Sci. 55(8); 4813-4822).
• Specificity: Clone 8.1.1
• Immunogen: Epitope: extracellular domain
• Application: Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in mouse lung tissue sections.Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Farr, A., et. al. (1992). J Histochem Cytochem. 40(5):651-64).Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Farr, A., et. al. (1992). J Histochem Cytochem. 40(5):651-64).Immunocytochemistry Analysis: A representative lot detected Podoplanin in Immunocytochemstry applications (Kamo, N., et. al. (2006). Am J Physiol Gastrointest Liver Physiol. 292(2):G526-34; Cohen, J.N., et. al. (2010). J Exp Med. 207(4):681-8; Ishii, T., et. al. (2010). Cell Tissue Res. 339(3):505-12).Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Farr, A., et. al. (1992). J Histochem Cytochem. 40(5):651-64).Immunoprecipitation Analysis: A representative lot immunoprecipitated Podoplanin in Immunoprecipitation applications (Farr, A., et. al. (1992). J Histochem Cytochem. 40(5):651-64).
• Quality: Evaluated by Immunohistochemistry (Paraffin) in mouse lung tissue sections.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Podoplanin in mouse lung tissue sections.
• Target description: 18.23 kDa calculated.
• Physical form: Format: Purified
• Storage and Stability: Stable for 1 year at 2-8°C from date of receipt.
• Other Notes: Concentration: Please refer to lot specific datasheet.
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