Anti-CEACAM1/CD66a Antibody, clone B3-17

Description

• General description: CEACAM1 (carcinoembryonic antigen-related cell adhesion molecule 1) is thought to be a member of the immunoglobulin superfamily and is known as an epithelial tumor suppressor and an angiogenic growth factor. It has also been linked to the actin-based cytoskeleton. CEACAM1 is also known as a cellular receptor for a number of human mucosa pathogenic bacteria. The loss of activity of CEACAM1 has been related to the development of colorectal cancer.
• Immunogen: Recombinant human CEACAM1/CD66a.
• Application: ELISA Analysis: 10 µg/mL from a representative lot detected human CEACAM1/CD66a by ELISA (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).Flow Cytometry Analysis: 10 µg/mL from a representative lot detected the exogenously expressed human CEACAM1/CD66a on the surface of transfected HeLa cells (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected CEACAM1/CD66a on the surface of HT-29 human colorectal carcinoma cells (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).Immunohistochemistry Analysis: 10 µg/mL from a representative lot detected CEACAM1/CD66a immunoreactivity in human jejunum tissue (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany). Western Blotting Analysis: 10 µg/mL from a representative lot detected the exogensously expressed human CEACAM1/CD66a extracellular domain-Fc fusion in lysates from transfected cells (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany). Flow Cytometry Analysis: Representative lots, either unconjugated or FITC-conjugated, detected CECAM1 immunoreactivity on the surface of human peripheral blood naïve and memory B-cells (Khairnar, V., et al. (2015). Nat. Commun. 6:6217; Seifert, M., et al. (2015). Proc. Natl. Acad. Sci. 112(6):E546-555).Flow Cytometry Analysis: A representative lot detected CEACAM1/CD66a induction on the surface of normal human bronchial epithelial (NHBE) cells stimulated with poly(I:C) or interferons (Klaile, E., et al. (2013) Respir Res. 14:85).Flow Cytometry Analysis: A representative lot detected CEACAM1/CD66a expression on the surface of 2 day-starved human epithelial (HT29, T102/3) and endothelial (AS-M.5) cells, as well as multivesicular bodies (MVBs) derived from these cells (Muturi H.T., et al. (2013) PLoS One. 8(9):e74654).Immunocytochemistry Analysis: A representative lot detected CEACAM1/CD66a immunoreactivity on the surface of normal human bronchial epithelial (NHBE) cells by indirect immunofluorescence staining (Klaile, E., et al. (2013) Respir Res. 14:85).Immunohistochemistry Analysis: A representative lot detected CEACAM1/CD66a in paraffin-embedded human lung cancer tissue sections (Klaile, E., et al. (2013) Respir Res. 14:85).Western Blot Analysis: A representative lot detected CEACAM1/CD66a in 2 day-starved AS-M.5 human endothelialn cells and AS-M.5-derived multivesicular bodies (MVBs) (Muturi H.T., et al. (2013) PLoS One. 8(9):e74654).
• Quality: Evaluated by Western Blotting in HepG2 cell lysate.Western Blotting Analysis: 2 µg/mL of this antibody detected CEACAM1/CD66a in 10 µg of HepG2 cell lysate.
• Target description: ~160 kDa observed. Target band size appears larger than the calculated molecular weights of 57.56/53,80 kDa (pro-/mature isoform 1; BGPa, CEACAM1-4L, TM1-CEA), 45.95/42.19 kDa (pro-/mature isoform 2; BGPg, CEACAM1-4C1), 35.30/31.54 kDa (pro-/mature isoform 3; BGPh, CEACAM1-3), 38.58/34.81 kDa (pro-/mature isoform 4; BGPi, CEACAM1-3C2), 50.35/46.58 kDa (pro-/mature isoform 5; BGPy, CEACAM1-3AL), 46.91/43.15 kDa (pro-/mature isoform 6; BGPb, CEACAM1-3L, TM2-CEA), 27.43/23.67 kDa (pro-/mature isoform 7; BGPx, CEACAM1-1L), 50.52/46.76 kDa (pro-/mature isoform 8; BGPc, CEACAM1-4S, TM3-CEA), 43.06/39.30 kDa (pro-/mature isoform 9; BGPz, CEACAM1-3AS), 51.15/47.38 kDa (pro-/mature isoform 10), 39.87/36.11 kDa ((pro-/mature isoform 11; BGPd, CEACAM1-3S) due to glycosylation. Uncharacterized bands may be observed in some lysates.
• Physical form: Format: Purified
• Other Notes: Concentration: Please refer to lot specific datasheet.