Anti-Twist1 antibody produced in rabbit

Description

• General description: Twist is encoded by the gene mapped to human chromosome 7p21.1. The encoded protein is a basic helix-loop-helix (bHLH) transcription factor. Twist 1 belongs to the Twist gene family and is mainly expressed in the suture mesenchyme.
• Specificity: Anti-Twist1 antibody recognizes human Twist1. Detection of the Twist1 band by immunoblotting is specifically inhibited with the immunizing peptide.
• Immunogen: synthetic peptide corresponding to amino acids 12-27 of human Twist1 with a C-terminal added cysteine, conjugated to KLH. The corresponding peptide sequence is identical in both rat and mouse.
• Application: Anti-Twist1 antibody is suitable for use in detecting Twist1 via in immunochemical applications such as immunoblotting (~26 kDa) and immunofluorescence.Immunoblotting: a working antibody concentration of 0.5-1 mg/mL is recommended using extracts of human 293T cells expressing recombinant human Twist1 and a chemiluminescent detection reagent.
• Biochem/physiol Actions: Twist is required for mesoderm formation. Twist 1 controls embryonic morphogenesis. It also plays a key role in metastasis by inducing an epithelial-mesenchymal transition (EMT). During an EMT, Twist stimulates the expression of mesenchymal markers, such as fibronectin and N-cadherin. In addition, it also blocks E-cadherin expression inhibiting cell-cell adhesion and therefore inducing infiltrative tumor growth in several human carcinomas. Twist1 is required in head mesenchyme for cranial neural tube morphogenesis in mice. Twist1 is involved in osteoblast differentiation and maturation. Increased expression of Twist 1 in breast cancer cells induce angiogenesis and leads to chromosomal instability. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome, an autosomal dominant defect characterized by minor skull and limb anomalies.
• Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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