Anti-Rac1 Antibody, clone 23A8 ZooMAb® Mouse Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 23A8 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects RAC1.
• Immunogen: Full-length recombinant human RAC1.
• Application: Quality Control TestingWestern Blotting Analysis: A 1:1,000 dilution of this antibody detected Rac1 in Rat Brain Microsomes.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Rac1 in A431, HeLa, and NIH3T3 cell lysates.Immunohistochemistry (Paraffin) Analysis: A 1:100 silution from a representative lot detected Rac1 in human esophagus tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rac1 in HeLa, A431, HUVEC , and NIH 3T3 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Ras-related C3 botulinum toxin substrate 1 (UniProt: P63000; also known as EC:3.6.5.2, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1) is encoded by the RAC1 (also known as TC25, MIG5) gene (Gene ID: 5879) in human. Rac1 is a plasma membrane-associated small GTPase that cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses. Two isoforms of Rac1 have been described that are produced by alternative splicing. Rac1b differs from Rac1a in that it has an additional 19 amino acids and is found in various tumors, such as breasts and colorectal. Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It can bind to the GTPase-binding domain of PAK, but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.