Anti-Nuclei Antibody, clone 3E1.3 ZooMAb® Mouse Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 3E1.3 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects human cell nuclei.
• Immunogen: Purified nuclei from human cells.
• Application: Quality Control TestingEvaluated by Immunohistochemistry (Paraffin) in human cerebral cortex tissue sections.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Nuclei in human cerebral cortex tissue sections.Tested applicationsImmunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Nuclei in human kidney tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nuclei in HeLa, A431 and HepG2 cells.Flow Cytometry Analysis: 1 μg from a representative lot detected Nuclei in one million HeLa cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: The nucleus is a membrane-enclosed organelle found in eukaryotic cells that contains the genetic information of the cell. In the nucleus the integrity of genes is maintained. Nuclear marker antibodies are useful tools to study the morphology and dynamics of the nucleus and provide a means for monitoring changes in nucleus during different phases of the cell cycle. Clone 3E1.3 is a mouse monoclonal antibody that serves as an excellent marker for nuclei in human cells, including neurons and embryonic stem cells. However, it does not react with rat or mouse cell nuclei. It gives a diffuse nuclear staining pattern. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.