Anti-CFTR Antibody, clone MM13-4 ZooMAb® Mouse Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone MM13-4 is a ZooMAb® Mouse recombinant monoclonal antibody that specifically detect CFTR. It targets an epitope within 12 amino acids from the N-terminal, first cytoplasmic domain.
• Immunogen: A linear peptide corresponding to 12 amino acids from the N-terminal, extracellular domain of human Cystic fibrosis transmembrane conductance regulator (CFTR).
• Application: Quality Control TestingEvaluated by Western Blotting in HT-29 cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CFTR in HT-29 cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected CFTR in CFTR transfected human bronchial epithelial cell lysate.Flow Cytometry Analysis: 0.1 μg from a representative lot detected CFTR in one million CFTR transfected human bronchial epithelial cells.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CFTR in CFTR transfected bronchial epithelial cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end userEvaluated by Western Blotting in HT-29 cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CFTR in HT-29 cell lysate.
• Target description: Cystic fibrosis transmembrane conductance regulator (UniProt: P13569; also known as CFTR, ATP-binding cassette sub-family C member 7, Channel conductance-controlling ATPase, cAMP-dependent chloride channel) is encoded by the CFTR (also known as ABCC7) gene (Gene ID: 1080) in human. CFTR is a multi-pass membrane protein with seven cytoplasmic domains, twelve transmembrane domains, and six extracellular domains. CFTR is an epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis. It mediates the transport of chloride ions across the cell membrane. This channel is also reported to be permeable to bicarbonate ions. Its channel activity is coupled to ATP hydrolysis. It is shown to play an important role in airway fluid homeostasis and contributes to the regulation of the pH and the ion content of the airway surface fluid layer and thereby has an important role in defense against pathogens. CFTR channel is internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane. Mutations in CFTR gene are reported to cause Cystic fibrosis that is characterized by chronic bronchopulmonary disease with recurrent respiratory infections and pancreatic insufficiency. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Shah, VS., et al. (2016). Science. 351(6272):503-507; Shcheynikov, N., et al. (2004). J. Biol. Chem. 279(21); 21857-21865).
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.