Anti-Poly(ADP-ribose) Antibody, clone 10H ZooMAb® Mouse Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 10H a ZooMAb® mouse recombinant monoclonal antibody that specifically detect Poly (ADP-Ribose) polymer.
• Immunogen: Poly(ADP-Ribose) polymer mixed with methylated bovine serum albumin.
• Application: Quality Control TestingEvaluated by Western Blotting in HeLa cell lysate.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Poly(ADP-ribose) in HeLa cell lysate.Tested ApplicationsImmunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Poly(ADP-ribose) in HeLa treated with 0.1% Hydrogen Peroxide, but not in untreated cells.Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated Poly(ADP-ribose) in HeLa treated with 0.1% Hydrogen Peroxide.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Poly(ADP-ribose) (PAR) that is synthesized from NAD+ by PAR polymerases (PARPs) is reported to regulate many physiological processes such as the maintenance of DNA integrity, gene expression and cell division. It is a unique post-translational modification that regulates cell survival and cell-death. This modification is brought about by the action of poly(ADP-ribose) polymerase (PARP), an abundant nuclear enzyme that uses nicotinamide adenine dinucleotide (NAD+). In humans, 17 different PARPs have been described, six of these add multiple ADP-ribose units and nine add only single units. Poly(ADP-ribose) contains a DNA-binding domain with two zinc fingers in its N-terminal domain, which is linked to the C-terminal NAD+-binding domain by a short region containing several glutamic acid residues that are sites of auto-poly(ADP-ribosyl)ation). Synthesis of poly(ADP-ribose) within the cell is initiated by agents that generate DNA strand interruptions. The branched homopolymer chains can reach a length of 200 to 300 residues but they are rapidly degraded after synthesis. Poly(ADP-ribose) are degraded by two classes of enzymes. The first enzyme cleaves ribose-ribose bonds between ADP-ribose subunits and the second enzyme removes the terminal ADP-ribose group from modified proteins. Poly(ADP-ribose) glycohydrolase (PARG) is the primary enzyme that cleaves ribose-ribose bonds. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Leung, AKL. (2014). J. Cell Biol. 205(5); 613-619; Schreiber, V., et al. (2006). Nat. Rev. Mol. Cell Biol. 7(7); 517-528).
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.