Anti-ATRX Antibody, clone 2A12, ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2A12 is a ZooMAb rabbit recombinant monoclonal antibody that detects Transcriptional regulator ATRX. It targets an epitope with in 141 amino acids from the C-terminal region.
• Immunogen: GST/His-tagged recombinant fragment corresponding to 141 amino acids from the C-terminal region of human Transcriptional regulator ATRX (isoform 4).
• Application: Anti-ATRX, clone 2A12, ZooMAb, Cat. No. ZRB001906, is a recombinant Rabbit Monoclonal Antibody that specifically detects ATRX and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: Transcriptional regulator ATRX (UniProt: P46100; also known as EC:3.6.4.12, ATP-dependent helicase ATRX, X-linked helicase II, X-linked nuclear protein, XNP, Znf-HX, ATRX) is encoded by the ATRX (also known as RAD54L, XH2) gene (Gene ID: 546) in human. ATRX is ubiquitously distributed and is involved in transcriptional regulation and chromatin remodeling. ATRX has a PHD zinc finger motif and an ATPase/helicase domain of the SWI/SNF type. It facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. ATRX binds to DNA tandem repeat sequences in both telomeres and euchromatin and is shown to bind quadruplex structures in vitro. ATRX associates with pericentromeric heterochromatin during interphase and mitosis, probably by interacting with CBX5/HP1 alpha. It interacts with DAXX to form the chromatin remodeling complex ATRX:DAXX, which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres. ATRX is believed to be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. ATRX is phosphorylated at serine residues during mitosis and phosphorylation is most evident at pro-metaphase stage. Phosphorylation of ATRX is necessary for release of the protein from the nuclear matrix and that it may facilitate progression to mitosis. Dephosphorylation of ATRX seems to coincide with exit of the cells from M phase. Mutations in ATRX gene are known to cause severe psychomotor and mental retardation, facial dysmorphism, and urogenital abnormalities. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Berube, NG et al. (2000). Hum. Mol. Gen. 9(4):539-547).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.