Anti-LC3B Antibody, clone 12K5, ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 12K5 is a ZooMAb rabbit recombinant monoclonal antibody that detects Microtubule-associated proteins 1A/1B light chain 3B (LC3B) in human and rat. It targets an epitope with in 14 amino acids from the N-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human Microtubule-associated proteins 1A/1B light chain 3B (LC3B).
• Application: Anti-LC3B, clone 12K5 ZooMAb, Cat. No. ZRB100, is a recombinant Rabbit Monoclonal Antibody that detects Microtubule-associated proteins 1A/1B light chain 3B and has been tested for use in Immunocytochemistry, Immunofluorescence,Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: Microtubule-associated proteins 1A/1B light chain 3B (UniProt: Q9GZQ8; also known as Autophagy-related protein LC3B, Autophagy-related ubiquitin-like modifier LC3B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3B, Microtubule-associated protein 1 light chain 3 beta) is encoded by the MAP1LC3B (also known as MAP1ALC3) gene (Gene ID: 81631) in human. LC3 is ubiquitously distributed in all eukaryotes and is expressed as three splice variants known as LC3A, LC3B, and LC3C. They possess a ubiquitin-like structure despite differences in their respective amino acid sequences. The amino-terminus of LC3 is composed of an alpha-helix that varies among the 3 members. However, its C-terminus is conserved and consists of a ubiquitin core composed of beta strands with hydrophobic pockets. The ubiquitin core region is responsible for its interaction with Atg core machinery proteins. LC3 is cleaved by Atg4 between amino acids 120-121 to form LC3-I and expose its C-terminal glycine. LC3-I then conjugates to phosphatidylethanolamine (PE) by Atg7, Atg3, and the Atg12 complex to generate LC3-II, which is inserted into autophagosome membrane. LC3B is reported to be most abundant in heart, brain, skeletal muscle and testis and only a trivial expression is observed in the liver. Higher expression of LC3B in triple negative breast cancer is associated with poor outcomes in patients. It plays a role in mitophagy and regulates mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess reactive oxygen species production. C3B is synthesized with a propeptide (aa 121-125), which is cleaved off to produce the mature form. LC3B can undergo phosphorylation at threonine 12 by protein kinase A (PKA) and this phosphorylation inhibits its conjugation to phosphatidylethanolamine. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lee, YK and Lee JA (2016). BMB Rep. 49(8): 424-430).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.