Anti-DOCK11 Antibody, clone 1F11 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1F11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects DOCK11 protein. It targets an epitope within 25 amino acids from the C-terminal half.
• Immunogen: KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal half of human DOCK11 protein.
• Application: Anti-DOCK11, clone 1F11 ZooMAb, Cat. No. ZRB1048, is a recombinant Rabbit monoclonal antibody that specifically targets DOCK11 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.
• Target description: Dedicator of cytokinesis protein 11 (UniProt: Q5JSL3; also known as Activated Cdc42-associated guanine nucleotide exchange factor, ACG, Zizimin-2, DOCK11) is encoded by the DOCK11 (also known as ZIZ2) gene (Gene ID: 139818) in human. DOCK11 is a guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. It is shown to be essential for marginal zone (MZ) B-cell development and associated with early bone marrow B-cell development, MZ B-cell formation, MZ B-cell number and marginal metallophilic macrophages morphology. DOCK11 contains DHR-1 (aa 640-880) and DHR-2 (aa 1609-2036) domains. The DHR-2 domain is essential for its GEF activity and mediates interaction with activated CDC42 in conjunction with residues 66-126. DOCK11 expression has been reported in Germinal center B lymphocytes, spleen, thymus, bone marrow, and in peripheral blood lymphocytes. Lower levels of expression have been observed in NIH-3T3 fibroblasts, 2C12 myoblasts and Neuro-2A neuroblastoma cells.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.