Anti-Insulin Antibody, clone 3B5 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 3B5 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects insulin.
• Immunogen: KLH-conjugated linear peptide corresponding to 14 amino acids from the b-chain of human insulin.
• Application: Quality Control TestingWestern Blotting Analysis: A 1:1,000 dilution of this antibody detected Insulin in Human Pancreas tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected recombinant human Insulin.Affinity Binding Assay: A representative lot of this antibody bound Insulin peptide with a KD of 6.2 x 10-8 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 Dilution from a representative lot detected Insulin in human pancreas tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Insulin in RIN-mF5 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Insulin (UniProt: P01308) is encoded by the INS gene (Gene ID: 3630) in human. Insulin is an anabolic hormone produced by the b-cells of pancreas. Its major effects are to increase cell permeability of monosaccharides, amino acids, and fatty acids. It accelerates glycolysis and glycogen synthesis in the liver and reduces gluconeogenesis. It is also shown to increase oxidation of substrates in the Kreb s cycle. It counters the concerted action of a number of hyperglycemia generating hormones. Action of insulin on target cells is mediated via its interaction with insulin receptor (IR), a heterotetrameric glycoprotein consisting of two extracellular -subunits (135 kDa) and two transmembrane -subunits (95 kDa). IR functions as an allosteric enzyme in which the -subunit inhibits the tyrosine kinase activity of the -subunit. Insulin binding to the -subunits results in the stimulation of the tyrosine kinase activity of the -subunits, which results in phosphorylation of insulin-receptor substrates to propagate insulin signaling and activation of downstream signaling molecules, including PI 3-kinase. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mohan, C., et al. (1989). Curr. Topics Cell Regul. 30; 105-142; Posner, BI. (2017). Can. J. Diabetes. 41(1); 108-113).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.