Anti-FAM49A Antibody, clone 2D6 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2D6 is ZooMAb rabbit recombinant monoclonal antibody that specifically detects CYFIP-related Rac1 interactor A (FAM49A). It targets an epitope within 24 amino acids from the N-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of human CYFIP-related Rac1 interactor A (FAM49A).
• Application: Quality Control TestingWestern Blotting Analysis: A 1:1,000 dilution of this antibody detected FAM49A in Human Hippocampus Membrane extractTested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected FAM49A in Human tonsil tissue lysate.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FAM49A in U2OS cells.Flow Cytometry Analysis: 1 μg from a representative lot detected FAM49A in one million U2OS cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FAM49A in human tonsil tissue sections.Affinity Binding Assay: A representative lot of this antibody bound FAM49A peptide with a KD of 5.8 x 10-7 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: CYFIP-related Rac1 interactor A (UniProt: Q9H0Q0; also known as Protein CYRIA, FAM49A) is encoded by the CYRIA (also known as FAM49A) gene (Gene ID: 81553) in human. FAM49A is a evolutionarily conserved protein that is suggested to negatively regulate RAC1 signaling and RAC1-driven cytoskeletal remodeling. It may also regulate chemotaxis, cell migration, and epithelial polarization by controlling the polarity, plasticity, duration and extent of protrusions. It binds to activated Rac1 via a domain of unknown function (DUF1394). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Whitelaw, JA., et al. (2019). Commun. Integr. Biol. 12(1); 112-118).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.