Anti-MBP Antibody, clone 2N6 , ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2N6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Myelin Basic Protein (MBP). It targets an epitope within 19 amino acids from the C-terminal half.
• Immunogen: KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human Myelin Basic Protein.
• Application: Anti-MBP, clone 2N6 ZooMAb, Cat. No. ZRB1219, is a highly specific recombinant rabbit monoclonal antibody that detects Myelin basic protein and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: Myelin basic protein (UniProt: P02686; also known as MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein) is encoded by the MBP gene (Gene ID: 4155) in human. MBP is a homodimeric protein that is found in both the central and the peripheral nervous system. It is one of the most abundant protein components of the myelin membrane in the CNS and plays a role in both the formation and stabilization of this myelin membrane. It is responsible for adhesion of the cytosolic surfaces of multi-layered compact myelin. MBP can interact with many polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and with negatively charged lipids. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. MBP is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. MBP expression begins abruptly in 14-16-week-old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane that can lead to demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating Marburg disease. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.