Anti-FcεRI γ-subunit Antibody, clone 1B6 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1B6 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects High affinity immunoglobulin epsilon receptor subunit gamma (FceRI g ). It targets an epitope within 19 amino acids from the C-terminal, cytoplasmic domain.
• Immunogen: KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human High affinity immunoglobulin epsilon receptor subunit gamma (FceRI g ).
• Application: Quality Control TestingEvaluated by Western Blotting in Mouse Spleen tissue lysate.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected FceRI g-subunit subunit in Mouse Spleen tissue lysate.Tested ApplicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected FceRI g-subunit in RBL and U-937 cell lysate.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FceRI g-subunit subunit in U-87 MG cell line.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected FceRI g-subunit in human spleen tissue sections.Affinity Binding Assay: A representative lot of this antibody bound FceRI g-subunit peptide with a KD of 3.0 x 10-8 in an affinity binding assay.Flow Cytometry Analysis: 0.1 μg from a representative lot detected FcERI y subunit in Human peripheral blood mononuclear cells (PBMC).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: High affinity immunoglobulin epsilon receptor subunit gamma (UniProt: P30273; also known as Fc receptor gamma-chain, FcRgamma, Fc-epsilon RI-gamma, IgE Fc receptor subunit gamma, FceRI gamma) is encoded by the FCER1G gene (Gene ID: 2207) in human. FceRI g is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that contains a short extracellular domain (aa 19-23), a transmembrane domain (aa 24-44), and a cytoplasmic domain (aa 45-86). FceRI g serves as an adapter protein that contains an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors. The g-subunit has a critical role in allowing the IgE Fc receptor to reach the cell surface. It associates with a variety of FcR alpha chains to form a functional signaling complex that regulates several aspects of the immune response. As a component of the high-affinity immunoglobulin E (IgE) receptor, it is shown to mediate allergic inflammatory signaling in mast cells and as a constitutive component of interleukin-3 receptor complex, it selectively mediates interleukin 4/IL4 production by basophils and primes T-cells toward effector T-helper 2 subset. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.