Anti-TFE3 Antibody, clone 1G5 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1G5 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Transcription Factor E3 (TFE3). It targets an epitope within the C-terminal half.
• Immunogen: His-tagged recombinant fragment corresponding to 143 amino acids from the C-terminal half of human Transcription factor E3 (TFE3).
• Application: Quality Control TestingEvaluated by Western Blotting in NIH3T3 cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TFE3 in NIH3T3 cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected TFE3 in HeLa and C6 cell lysates.Affinity Binding Assay: A representative lot of this antibody bound Recombinant TFE3 Protein with a KD of 1.6 x 10-8 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TFE3 in human testis tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TFE3 in PC3 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Transcription factor E3 (UniProt: P19532; also known as Class E basic helix-loop-helix protein 33, bHLHe33, TFE3) is encoded by the TFE3 (also known as BHLHE33) gene (Gene ID: 7030) in human. TFE3 is a ubiquitously expressed transcription factor that acts as a master regulator of lysosomal biogenesis and immune response. It specifically recognizes and binds E-box sequences (5′-CANNTG-3′) and its efficient DNA-binding requires homodimerization or heterodimerization with another member of the MiT/TFE family, such as TFEB or MITF. TFE3 is reported to be involved in the cellular response to amino acid availability by acting downstream of MTOR. In the presence of nutrients, it undergoes phosphorylation by mTOR that promotes its cytosolic retention and subsequent inactivation. However, under conditions of starvation or lysosomal stress, inhibition of mTOR induces TFE3 dephosphorylation that results in its nuclear localization and transcription factor activity. TFE3 is also reported to act as a positive regulator of browning of adipose tissue by promoting expression of target genes. It also plays a role in maintenance of the pluripotent state of embryonic stem cells by promoting the expression of genes such as ESRRB. Its mTOR-dependent nuclear exclusion promotes exit of cells from pluripotency. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.