Anti-ASTN2 Antibody, clone 2E8 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2E8 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Astrotactin-2. It targets an epitope within 18 amino acids from the C-terminal half, lumenal domain.
• Immunogen: KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal half, lumenal domain.
• Application: Quality Control TestingEvaluated by Western Blotting in Mouse brain membrane preparations.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected ASTN2 in Mouse brain membrane preparations.Tested ApplicationsAffinity Binding Assay:: A representative lot of this antibody bound ASTN2 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.Flow Cytometry Analysis: 1 μg from a representative lot detected ASTN2 in one million A431 cells.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ASTN2 in A431 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Astrotactin-2 (UniProt: O75129; also known as ASTN2) is encoded by the ASTN2 (also known as KIAA0634) gene (Gene ID: 23245) in human. Astrotactin-2 is a multi-pass membrane protein that is synthesized with a signal peptide (aa 1-49), which is subsequently cleaved off and the mature form has two lumenal domains (aa 50-206 and 456-1339), two transmembrane domains (aa 207-227 and 435-455) and one cytoplasmic domain (aa 228-434). It also contains three EGF-like domains and one fibronectin type III domain. Its highest expression is observed in the cerebellum and lower levels of expression are shown in hippocampus, cortex, and olfactory bulb. It localizes mostly to vesicles inside neural cells that may give a punctate antibody staining pattern in the soma and along neuronal processes. Astrotactin-2 interacts with Astrotactin-1 and mediates its recycling to the anterior pole of the cell membrane in migrating neurons. It is shown to be a key regulator of dynamic trafficking of synaptic proteins. It associates with recycling and degradative vesicles in cerebellar neurons and promotes endocytic trafficking and degradation of synaptic proteins. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Behestia, H., et al. (2018). Proc. Natl. Acad. Sci. USA. 115(41); E9717 E9726; Ni, T. et al (2016). Open Biol. 6; 160053).
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.