Anti-LONP1 Antibody, clone 1I18, ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1I18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Mitochondrial ATP-dependent protease Lon (LONP).
• Immunogen: GST/His-tagged recombinant fragment corresponding to 149 amino acids from the central region of Human LONP1.
• Application: Anti-LONP1, clone 1I18 ZooMAb, Cat. No. ZRB1314, is a recombinant rabbit monoclonal antibody that specifically detects mitochondrial Lon protease homolog (LONP1) and has been tested for use in Immunocytochemistry, and Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: Lon protease homolog, mitochondrial (UniProt: P36776; also known as EC:3.4.21.53, LONHs, Lon protease-like protein, LONP, Mitochondrial ATP-dependent protease Lon, Serine protease 15) is encoded by the LONP1 (also known as PRSS15) gene (Gene ID: 9361) in human. LONP is a homohexameric, nuclear encoded, mitochondrial matrix protein that acts as an ATP-dependent serine protease. It is expressed ubiquitously and is present at high levels in the liver, brain, heart, skeletal muscle, placenta. It is synthesized with a transit peptide (aa 1-67) that helps in its import into the matrix where it is cleaved off to generate the mature form. It is organized in a ring with a central cavity and the ATP-binding and proteolytic domains (AP-domain) form a hexameric chamber. Its N-terminal domain (aa 124-368) is crucial for the overall structure of the protein, maintaining it in a conformation allowing its proper functioning. It is highly conserved throughout evolution and is shown to be crucial for the maintenance of mitochondrial homeostasis. LONP mediates the selective degradation of mutant and abnormal proteins in mitochondria. It also has a chaperone function in the assembly of inner membrane protein complexes. It also participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial DNA. Its DNA and RNA binding is stimulated by substrate and inhibited by ATP binding. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pinti, M., et al. (2016). Biochim. Biophys. Acta 1857(8); 1300-1306).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.