Anti-THSD7A Antibody, clone 2F15-L1 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2F15-L1 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Thrombospondin type-1 domain-containing protein 7A (THSD7A). It targets an epitope within 20 amino acids from the extracellular domain within the N-terminal half.
• Immunogen: KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal half of human Thrombospondin type-1 domain-containing protein 7A (THSD7A).
• Application: Quality Control TestingEvaluated by Western Blotting in Human kidney tissue lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected THSD7A in Human kidney tissue lysate.Tested ApplicationsImmunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected THSD7A in Human kidney tissue sections.Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected THSD7A in Human kidney tissue sections.Affinity Binding Assay: A representative lot of this antibody bound THSD7A peptide with a KD of 9.8 x 10-8 in an affinity binding assay.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected THSD7A in Human umbilical vein endothelial cells (HUVEC).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Thrombospondin type-1 domain-containing protein 7A (UniProt: Q9UPZ6; also known as THSD7A) is encoded by the THSD7A (also known as KIAA0960) gene (Gene ID: 221981) in human. THSD7A is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-47), which is subsequently cleaved to produce the mature form that contains an extracellular domain (aa 48-1607), a transmembrane domain (aa 1608-1628), and a short cytoplasmic domain (aa 1629-1657). It contains 19 thrombospondin type 1 repeats, multiple WSXW motifs, and one RGD motif (aa 1568-1570). It is detected on kidney podocytes along the glomerular capillary wall. It plays a role in actin cytoskeleton rearrangement. There also exists a soluble form that promotes endothelial cell migration and filopodia formation during sprouting angiogenesis via a focal adhesion kinase-dependent mechanism. THSD7A is a well-known antigenic target of autoantibodies leading to primary membranous glomerulopathy and accounts for about 10% cases of phospholipase A2 receptor-negative membranous glomerulopathy. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tomas,NM., et al. (2016). J. Clin. Invest. 26(7); 2519-2532; Kuo, MW., et al. (2011). PLoS One. 6(12); e29000).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.