Anti-PEA15 Antibody, clone 2J13 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2J13 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Astrocytic phosphoprotein PEA-15.
• Immunogen: GST/His-tagged full-length recombinant human Astrocytic phosphoprotein PEA-15.
• Application: Anti-PEA15, clone 2J13 ZooMAb, Cat. No. ZRB1444, is a recombinant Rabbit monoclonal antibody that specifically targets PEA15 and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: Astrocytic phosphoprotein PEA-15 (UniProt: Q15121; also known as 15 kDa phosphoprotein enriched in astrocytes, Phosphoprotein enriched in diabetes, PED) is encoded by the PEA15 gene (Gene ID: 8682) in human.PEA-15 is a cytoplasmic protein that is ubiquitously expressed. Higher expressions are found in heart, brain, muscle, and adipose tissue. Higher expression of PEA-15 has been reported in tissues from individuals with type 2 diabetes. PEA-15 blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. It is reported to Inhibit RPS6KA3 activities by retaining it in the cytoplasm. It is also shown to inhibits both TNFRSF6- and TNFRSF1A-mediated caspase 8 activity and apoptosis. It also regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface. PEA-15 is phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones. PEA-15 has two microtubule binding sites (aa 98-107 and 122-129). This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in lyophilized from PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.