Anti-TIGIT Antibody, clone 1D20 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1D20 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human T-cell immunoreceptor with Ig and ITIM domains (TIGIT). It targets an epitope within 16 amino acids from the extracellular domain.
• Immunogen: KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal, extracellular domain of human T-cell immunoreceptor with Ig and ITIM domains (TIGIT).
• Application: Anti-TIGIT, clone 1D20 ZooMAb, Cat. No. ZRB1454, is a recombinant Rabbit monoclonal antibody that specifically targets TIGIT and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
• Target description: T-cell immunoreceptor with Ig and ITIM domains (UniProt: Q495A1; also known as V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3, TIGIT) is encoded by the TIGIT (also known as VSIG9, VSTM3) gene (Gene ID: 201633) in human. TIGIT is a single-pass type I membrane protein that binds with high affinity to the poliovirus receptor (PVR) forming a heterotetrameric assembly of two TIGIT and two PVR molecules. This causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. It is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells. It is also expressed on many cell types important in cancer, and its mechanism of action may depend on the cell type studied. TIGIT also serves as a functional ligand and induces dendritic cells to acquire a tolerogenic phenotype via ligation of PVR. TIGIT -/- mice, when challenged, develop severe experimental autoimmune encephalomyelitis compared to wild-type mice. TIGIT is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 22-141), a transmembrane domain (aa 142-162), and a cytoplasmic domain (aa 163-244). The cytoplasmic domain contains an immunoreceptor tyrosine-based inhibitor motif (ITIM; aa 229-234), which is involved in the modulation of cellular responses. The IgV domain (aa 22-124) of TIGIT possesses distinctive (V/I)(S/T)Q, AX6G, and T(F/Y)PX1G sub-motifs that mediate a ′lock and key′ trans-interaction with cis-homodimers of PVR. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Manieri, NA., et al. (2017). Trends Immunol. 38(1); 20-28).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.