Anti-HCF-1 Antibody, clone 1B17 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1B17 is a ZooMAb® rabbit recombinant monoclonal antibody that detects Host cell factor 1. It targets an epitope within 23 amino acids from the C-terminal, chain-6 region. It detects all C-terminal cleavage chains.
• Immunogen: KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal, chain-6 region of human Host cell factor 1 (HCF-1).
• Application: Quality Control TestingEvaluated by Western Blotting in HeLa cell lysate.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected HCF-1 in HeLa cell lysate.Tested applicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected HCF-1 in lysates from rat brain, A549 cells and NIH3T3 cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected HCF-1 in human colon tissue sections.Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected HCF-1 in NIH3T3 cells.Affinity Binding Assay: A representative lot of this antibody bound HCFC1 with a KD of 6.1 x 10-7 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Host cell factor 1 (UniProt: P51610; also known as HCF, HCF-1, C1 factor, CFF, VCAF, VP16 accessory protein) is encoded by the HCFC1 (also known as HCF1, HFC1) gene (Gene ID: 3054) in human. HCF-1, a cellular transcription factor and co-activator that is required for progression through the G1 phase of the cell cycle. It is a heterodimeric complex that contains six N-terminal and six C-terminal chains that remain tightly associated in a non-covalent manner. It also contains eight HCF repeats that are specific proteolytic cleavage signals. This complex is proteolytically cleaved into one or several PPCE THET sites within the HCF repeats. HCF-1 does not bind to DNA directly, however, both N- and C-terminal chains are shown to associate with DNA. The Kelch domains in the N-terminal region recognize and associate with HCF-binding motif present in many sequence-specific DNA-binding factors. HCF-1 is reported to act in concert with E2F1 to activate transcription of S phase genes. It recruits KMT2E/MLL5 to E2F1 responsive promoters that facilitate transcriptional activation and G1 to S phase transition. HCF-1 is highly expressed in fetal tissues and the adult kidney. However, its expression is reported in many tested tissues. Mutations in HCFC1 gene have been linked to X-linked 3 mental retardation. In addition, a single point mutation in the Kelch domain (P134S) is reported to cause a temperature-induced cell-proliferation arrest and cytokinesis defects. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhou, P., et al. (2013). J. Biol. Chem. 288(24); 17532-17543; Zargar, ZU, and Tyagi, S. (2012). Transcription. 3(4); 187-192; Tyagi, S., et al. (2007). Mol. Cell. 27(1); 107-119; Vogel, JL., and Kristie, TM. (2000). Proc. Natl. Acad. Sci. USA. 97(17); 9425-9430).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.