Anti-HDAC4 Antibody, clone 1C16 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1C16 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Histone deacetylase 4 (HDAC4). It targets an epitope within the first 19 amino acids from the N-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to the first 19 amino acids from the N-terminal region of human Histone deacetylase 4 (HDAC4).
• Application: Quality Control TestingEvaluated by Western Blotting in NIH3T3 cell lysate.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected HDAC4 in NIH3T3 cell lysate.Tested applicationsImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected HDAC4 in HeLa cells.Affinity Binding Assay: A representative lot of this antibody bound HDAC4 peptide with a KD of 2.8 x 10-8 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Histone deacetylase 4 (UniProt: P56524; also known as EC:3.5.1.98, HD4) is encoded by the HDAC4 (also known as KIAA0288) gene (Gene ID: 9759) in human. HDAC4 is a ubiquitous enzyme that is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. It is present as a homodimeric protein that shuttles between the nucleus and the cytoplasm. Its nuclear export sequence is localized in amino acids 1051-1084. Its export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein. 14-3-3 family of proteins are known to bind specifically to conserved phosphoserine-containing motifs. Phosphorylation of specific amino acids creates binding sites for the 14-3-3, which escorts phospho- HDAC4 from the nucleus to the cytoplasm. Phosphorylation of HDAC4 at serine 246, 467, and 632 is achieved by CaMK4. Its nuclear localization probably depends on sumoylation on lysine 559, which is promoted by E3 SUMO-protein ligase RanBP2. Two isoforms of HDAC4 have been described that are produced by alternative splicing. Isoform 2 lacks the first 117 amino acids. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wang, Z., et al. (2014). Epigenomics 6(1); 139-150; Wang, AH., et al. (2000). Mol. Cell Biol. 20(18); 6904-6912).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.