Anti-FOXO3A Antibody, clone 1G18 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1G18 is a Rabbit recombinant monoclonal antibody that specifically detects Forkhead box protein O3 (FOXO3A). It targets an epitope within 20 amino acids from the C-terminal half.
• Immunogen: KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human Forkhead box protein O3 (FOXO3A).
• Application: Quality Control TestingEvaluated by Western Blotting in MCF-7 cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FOXO3A in MCF-7 cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected FOXO3A in SH-SY5Y cell lysate.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected FOXO3A in human colon tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot from a representative lot detected FOXO3A in SH-SY5Y cells. Affinity Binding Assay: A representative lot of this antibody bound FOXO3A with a KD of 3.0 x 10-7 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Forkhead box protein O3 (UniProt: O43524; also known as AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1) is encoded by the FOXO3 (also known as FKHRL1, FOXO3A) gene (Gene ID: 2309) in human. Forkhead box protein O3 is a transcriptional activator that triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. It recognizes and binds to the DNA sequence 5′-[AG]TAAA[TC]A-3′. Its DNA binding domain located in amino acids 157-251. The nuclear localization signal is localized in amino acids 242-259. In the cytoplasm it is present in an inactive state and it translocates to the nucleus upon oxidative stress and in the absence of survival factors. In response to metabolic stress, it translocates into the mitochondria where it promotes mtDNA transcription. In the presence of survival factors such as IGF-1, it is phosphorylated on threonine 32 and serine 253 by AKT1/PKB. The phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes its translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. FOXO3 deacetylation by SIRT1 or SIRT2 stimulates its interaction SKP2 and facilitates SCF(SKP2)-mediated FOXO3 ubiquitination and proteasomal degradation. A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Peserica, A., et al. (2013). Cell Mol. Life Sci. 70(11); 2015-2029; Brunet, A., et al. (1999). Cell. 96(6); 857-868).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.