Anti-S100-B Antibody, clone 8F20 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 8F20 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects S100-B.
• Immunogen: His-tagged full-length recombinant human S100-B protein.
• Application: Quality Control TestingWestern Blotting Analysis: A 1:10,000 dilution of this antibody detected S100-B in Rat Brain tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected S100-B in mouse spinal cord, human brain, and A375 lysates.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected S100-B in human cerebellum tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected S100-B in A375 cells.Flow Cytometry Analysis: 1 μg from a representative lot detected S100-B in one million C6 cells.Affinity Binding Assay: A representative lot of this antibody bound S-100 B protein with a KD of 3.2 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Protein S100-B (UniProt: P04271; also known as S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B) is encoded by the S100B gene (Gene ID: 6285) in human. S100-B is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. It exists in both heterodimeric and homodimeric forms. Existence of dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain has been reported and dimerization seems to be essential for its biological activities. S100-B has been suggested to play a part in a variety of cellular processes, primarily via binding to key synaptic proteins and inhibiting their phosphorylation. The extracellular form of S100-B, secreted by astrocytes, is physiologically involved in the development and maintenance of CNS homeostasis. S100-B effects are closely dependent on its cellular levels. At nanomolar concentrations it is shown to enhance survival of neurons during development and stimulate neurite outgrowth in cerebral cortex neurons. It also reduces the loss of mitochondrial function and cell death. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Donato, R. (2001). Int. J. Biochem. Cell Biol 33(7); 637-668; Rezaei, O., et al. (2017). Iran J. Neurol. 16(2); 83-89).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.