Anti-NOTCH2 Antibody, clone 1B14-5 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1B14-5 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects NOTCH2. It targets an epitope within 18 amino acids from the C-terminal, cytoplasmic domain.
• Immunogen: KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal, cytoplasmic domain of human NOTCH2.
• Application: Quality Control TestingEvaluated by Western Blotting in HeLa cell lysate.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected NOTCH2 in HeLa cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected NOTCH2 in NIH3T3 and C6 cell lysates.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected NOTCH2 in human kidney tissue sections.Affinity Binding Assay: A representative lot of this antibody bound Notch2 peptide with a KD of 1.3 x 10-6 in an affinity binding assay.Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected NOTCH2 in MCF7 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Neurogenic locus notch homolog protein 2 (UniProt: Q04721; also known as Notch 2, hN2, NOTCH2) is encoded by the NOTCH2 gene (Gene ID: 4853) in human. Notch is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to generate the mature form that contains a long extracellular domain (aa 26-2677), a transmembrane domain (aa 1678-1698), and a cytoplasmic domain (aa 1699-2471). It is evolutionarily conserved and is implicated in cell-fate determination, tissue patterning, morphogenesis, and cell differentiation. It is expressed in the brain, heart, kidney, lung, skeletal muscle and liver and is ubiquitously expressed in the embryo. Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. It serves as a receptor for Jagged-1, Jagged-2, and Delta-1 to regulate cell-fate determination. Following ligand binding, Notch is cleaved by TNF-alpha converting enzyme (S2 cleavage) to yield a membrane-associated intermediate fragment called notch extracellular truncation, which is then cleaved by g-secretase (S3 cleavage) to release a notch-derived peptide containing the intracellular domain (NICD; aa 1697-2471) from the membrane that translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Yin, L., et al (2010). Biochem. Pharmacol. 80(5); 690-701).
• Physical form: Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.