Anti-GATM/AGAT Antibody, clone 1F4 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1F4 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Glycine amidinotransferase, mitochondria (GATM/AGAT). It targets an epitope within 22 amino acids from the N-terminal half.
• Immunogen: KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal half of human Glycine amidinotransferase, mitochondrial (GATM/AGAT).
• Application: Quality Control TestingEvaluated by Western Blotting in Rat kidney tissue lysate.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected GATM/AGAT in Rat Kidney tissue lysate.Tested ApplicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected GATM/AGAT in Human Kidney and Mouse Kidney tissue lysates.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GATM/AGAT in Human umbilical vein endothelial cells (HUVEC).Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected GATM/AGAT in human kidney tissues sections.Affinity Binding Assay: A representative lot of this antibody bound GATM/AGAT peptide with a KD of 1.2 x 10-6 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GATM/AGAT in human kidney tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Glycine amidinotransferase, mitochondrial (UniProt: P50440; also known as EC:2.1.4.1, L-arginine:glycine amidinotransferase, Transamidinase) is encoded by the GATM (also known as AGAT) gene (Gene ID: 2628) in human. GATM is a homodimeric protein that catalyzes the rate limiting step in the biosynthesis of guanidinoacetate, the immediate precursor of creatine. It displays an equilibrium between the monomeric and dimeric forms. It is synthesized with a mitochondrial transit peptide (aa 1-37), which is subsequently cleaved off to produce the active enzyme (aa 38-423). Its expression is observed in various tissues, with the highest expression observed in kidney. Its expression is elevated in the myocardium during cardiac failure. It may play a role in embryonic and central nervous system development and may also be involved in response to cardiac failure by elevating local creatine synthesis. Two isoforms of GATM have been described. Isoform 1 is localized in the mitochondrial inner membrane and isoform 2 is cytoplasmic. Mutations in GATM gene have been linked to cerebral creatine deficiency syndrome 3, an autosomal recessive disorder that is characterized by developmental delay, intellectual disability, and myopathy characterized by muscle weakness and atrophy. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Cullen, ME., et al. (2006). Circulation. 114(1 suppl); 116-120; Gross, MD., et al. (1986). Arch. Biochem. Biophys. 251(2); 747-755).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.