Anti-SIGLEC15 Antibody, clone 1B10 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1B10 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Sialic acid-binding Ig-like lectin 15 (SIGLEC15). It targets an epitope within 24 amino acids from the C-terminal, cytoplasmic domain.
• Immunogen: KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal, cytoplasmic domain of human Sialic acid-binding Ig-like lectin 15 (SIGLEC15).
• Application: Quality Control TestingEvaluated by Western Blotting in Raji cell lysate.Western Blotting Analysis (WB): A 1:10,000 Dilution of this antibody detected SIGLEC15 in Raji cell lysate. Tested ApplicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected SIGLEC15 in Human Tonsil tissue lysate.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SIGLEC15 in Raji cells.Flow Cytometry Analysis: 0.1 μg from a representative lot detected SIGLEC15 in one million Raji cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SIGLEC15 in human kidney tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Sialic acid-binding Ig-like lectin 15 (UniProt: Q6ZMC9; also known as Siglec-15, CD33 antigen-like 3) is encoded by the SIGLEC15 (also known as :CD33L3) gene (Gene ID: 284266) in human. SIGLEC15 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 20-263), a transmembrane domain (aa 264-284), and a cytoplasmic domain (aa 295-328). Its expression is observed in macrophages and in dendritic cells of spleen and lymph nodes. It serves as an immune checkpoint molecule and a critical immune suppressor. It suppresses antigen-specific T cell responses in vitro and in vivo. Genetic ablation or antibody blockade of SIGLEC15 is reported to amplify anti-tumor immunity in the tumor microenvironment and inhibits tumor growth in murine models. SIGLEC15-deficient mice also exhibit decreased serum IL-10 levels. Two isoforms of SIGLEC15 are described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wang, J., et al. (2019). Nat. Med. 25; 656-666).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.