Anti-Bid Antibody, clone 1G3 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1G3 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Bid.
• Immunogen: His-tagged, full-length, recombinant human Bid.
• Application: Quality Control TestingEvaluated by Western Blotting in HeLa cell lysate.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Bid in HeLa cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:19,000 dilution from a representative lot detected Bid in lysate from MCF-7 cells and 1:1,000 dilution in lysates from C2C12, and PC12 cells.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Bid in Human tonsil tissue sections.Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Bid in Human tonsil tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: BH3-interacting domain death agonist (UniProt: P55957; also known as p22 BID, BID) is encoded by the BID gene (Gene ID: 637) in human. Bid is a BH3 domain containing member of the Bcl-2 family  with proapoptotic function. It is shown to be crucial for death receptor-mediated apoptosis in many cell systems where it counters the protective effects of Bcl-2. It links the death receptor signaling pathway to the mitochondrial signaling pathway mediated by Bcl-2 proteins. Full length Bid that is localized in the cytoplasm is cleaved by caspase-8 to generate a truncated form (tBid) that translocates to mitochondria. tBid then binds to mitochondrial Bak and induces the release of cytochrome c. TNFα-induced caspase-mediated cleavage of Bid produced p15, 13, and p11 fragments.  Caspase-8-mediated Bid processing of Bid bridges the extrinsic and intrinsic apoptosis pathways that provide a mechanism to amplify death signals.  The BH3 motif of Bid is localized in amino acids 86-100. The hydrophobic FACE® of the BH3 domain, which is exposed in other family members, is enclosed by the N-terminal of Bid and only exposed following proteolytic cleavage of Bid. A second functional domain in Bid known as the BH3B domain, which is localized in the N-terminus region, is reported to suppress the apoptogenic activity of the BH3 domain. The region between these two opposing domains is sensitive to proteolytic cleavage and contains cleavage sites for caspases and granzyme B. Four isoforms produced by Alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity™, reproducibility, and stability over conventional monoclonals. (Ref.: Renshaw, SA.,  et al. (2004). J. Biol. Chem. 279(4); 2846-2855; Esposti, MD. (2002). Apoptosis. 7; 433-440).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Legal Information: Affinity is a trademark of Mine Safety Appliances Co.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.