Anti-CCR4/CD194 Antibody, clone 1N13 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1N13 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects C-C chemokine receptor type 4 (CCR4/CD194). It targets an epitope within 25 amino acids from the N-terminal, extracellular domain.
• Immunogen: KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal, extracellular domain of human C-C chemokine receptor type 4 (CCR4/CD194).
• Application: Quality Control TestingEvaluated by Western Blotting in K562 cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CCR4/CD194 in K562 cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected CCR4/CD194 in EL4 cell lysates.Affinity Binding Assay Analysis: A representative lot of this antibody bound CCR4-CD194 with a KD of 6.9 x 10^-8 in an affinity binding assay.Flow Cytometry Analysis: 0.1 µg from a representative lot detected CCR4/CD194 in one million Human peripheral blood mononuclear cells (PBMC).Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CCR4/CD194 in Jurkat cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CCR4/CD194 in Human spleen tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: C-C chemokine receptor type 4 (UniProt: P51679; also known as C-C CKR-4, CC-CKR-4, CCR4, K5-5, CD194) is encoded by the CCR4 (also known as CMKBR4) gene (Gene ID: 1233) in human. CCR4, a member of the G-protein coupled receptor 1 family, is a multi-pass membrane glycoprotein with seven transmembrane domains. It serves as a high affinity receptor for the C-C type chemokines CCL17 and CCL22, and CKLF1. It is predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets. Higher expression of CCR4 is also observed in Th2 cells, cutaneous lymphocyte antigen-positive skin-homing T cells, and Treg cells. It is also expressed in brain microvascular and coronary artery endothelial cells. Its levels are upregulated within hours of positive selection signaling and promotes medullary entry and clonal deletion of immature post-positive selection thymocytes. Higher expression of CCR4 is reported in adult T-cell leukemia and human T-cell leukemia virus type 1 (HTLV-1) immortalized T cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bogacka, J., et al. (2022), Int. J. Mol. Sci. 2022, 23(24), 15638; Osamu Yoshie, O., and Matsushima, K. (2015). Int. Immunol. 27(1); 11-20; Yoshie, O. (2002). Blood. 99(5); 1505-1511).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.