RAB0418

Human Ubiquitin+1 ELISA Kit

for serum, plasma, cell culture supernatant and urine

Manufacturer: Sigma Aldrich

Select a Size

Pack Size SKU Availability Price
1 KIT RAB0418-1-KIT In Stock ₹ 76,345.80

RAB0418 - 1 KIT

₹ 76,345.80

In Stock

Quantity

1

Base Price: ₹ 76,345.80

GST (18%): ₹ 13,742.244

Total Price: ₹ 90,088.044

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitablecapture ELISA: suitable

input

sample type plasmasample type cell culture supernatant(s)sample type serumsample type urine

assay range

inter-assay cv: <12%intra-assay cv: <10%sensitivity: 30 pg/mLstandard curve range: 27.43-20000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... RPS27A(6233)

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Description

  • General description: The Human Ubiquitin+1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Ubiquitin+1 in serum, plasma, cell culture supernatants and urine.The RPS27A (ribosomal protein S27a) gene is mapped to human chromosome 2p16.1. The protein complex encoded contains ubiquitin at the N-terminus and a C-terminal ribosomal protein S27a.[1]
  • Immunogen: Recombinant Human Ubiquitin
  • Application: For research use only. Not for use in diagnostic procedures.Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
  • Biochem/physiol Actions: Ribosomal protein S27a (RPS27a), also known as ubiquitin carboxyl extension protein 1 (UBCEP-1), is involved in ribosome biogenesis and post-translational modifications of proteins. The encoded protein enhances cell proliferation, controls cell cycle progression and inhibits apoptosis of leukemia cells. Overexpression of RPS27A results in advanced-phase chronic myeloid leukemia (CML) and acute leukemia (AL). Hence drugs targeting RPS27a combining with tyrosine kinase inhibitor (TKI) might be a potential treatment for TKI resistant CML patients.[2] Elevated expression of UBCEP-1, which is a component of ubiquitin pathway, promotes prostate carcinoma development, via increasing the degradation of proteins involved in growth inhibition or apoptosis.[3] RPS27a might act as a unique stress sensor in cells, which results in increased p53 activity leading to inhibition of cell cycle progression.[4]
  • Other Notes: A sample Certificate of Analysis is available for this product.Please type the word sample in the text box provided for lot number.

SAFETY INFORMATION

Pictograms

GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

WGK

WGK 3

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species reactivity:
human

packaging:
kit of 96 wells (12 strips x 8 wells)

technique(s):
ELISA: suitablecapture ELISA: suitable

input:
sample type plasmasample type cell culture supernatant(s)sample type serumsample type urine

assay range:
inter-assay cv: <12%intra-assay cv: <10%sensitivity: 30 pg/mLstandard curve range: 27.43-20000 pg/mL

detection method:
colorimetric

shipped in:
wet ice

storage temp.:
−20°C

Gene Information:
human ... RPS27A(6233)

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−20°C

Gene Information:
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