Coomassie Brilliant blue G 250 (C.I. 42655)
for electrophoresis
Manufacturer: Sigma Aldrich
CAS Number: 6104-58-1
Synonym(S): Coomassie Brilliant blue G 250 (C.I. 42655), Acid blue 90, Brilliant Blue G, Cyanine G, Polar Blue G, SERVA BLUE G, protein gel stain
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25 G | 1.15444-25-G | In Stock | ₹ 9,860.00 |
1.15444 - 25 G
In Stock
Quantity
1
Base Price: ₹ 9,860.00
GST (18%): ₹ 1,774.80
Total Price: ₹ 11,634.80
Quality Level
100
potency
>5000 mg/kg LD50, oral (Rat)
technique(s)
protein staining: suitable
pH
6.4 (20 °C, 10 g/L in H2O)
solubility
40 g/L
bulk density
520 kg/m3
storage temp.
no temp limit
InChI
1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1
InChI key
CMGWKYXJIYYESN-UHFFFAOYSA-M
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Description
- General description: Coomassie Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye.[1] CBB dyes are widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.[2] CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.[1]
- Application: Coomassie Brilliant blue G 250 (C.I. 42655) has been used to stain gel in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.[3] It has also been used in Bradford protein assay.[4]
- Analysis Note: Identity (UV/VIS-Spectrum): passes testAbsorption maximum λmax. (buffer pH 7.0): 577 - 584 nmSpec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; buffer pH 7.0; calc. on dried substance): 450 - 570TLC-Test: passes testLoss on drying (110 °C): ≤ 8 %Suitability for electrophoresis: passes test
SAFETY INFORMATION
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Quality Level: 100
potency: >5000 mg/kg LD50, oral (Rat)
technique(s): protein staining: suitable
pH: 6.4 (20 °C, 10 g/L in H2O)
solubility: 40 g/L
bulk density: 520 kg/m3
storage temp.: no temp limit
InChI: 1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1
InChI key: CMGWKYXJIYYESN-UHFFFAOYSA-M
Quality Level: 100
potency: __
technique(s): thin layer chromatography (TLC): suitable
pH: __
solubility: __
bulk density: __
storage temp.: 2-30°C
InChI: __
InChI key: __
Quality Level: 100
potency: __
technique(s): ICP: suitable
pH: 0.1 (20 °C in H2O)
solubility: __
bulk density: __
storage temp.: 15-25°C
InChI: __
InChI key: __
