Monoclonal ANTI-FLAG® antibody produced in rabbit

Description

• General description: Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG® peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in association with other tags. The small size of the FLAG tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function. The N-terminal FLAG® peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage catalyzed by Cu2+ ions of the C-terminal FLAG® peptide from a fusion protein has been reported. A sequence motif with five out of eight amino acid residues identical to the FLAG® peptide is found in both rat and mouse Mg2+ dependent protein b-phosphatase, as well as in the human and bovine enzyme. This rabbit ANTI-FLAG® antibody is produced from the hybridoma SIG1-25. The hybridoma is derived by the fusion of myeloma cells and splenocytes from rabbits immunized with the FLAG® peptide sequence conjugated to KLH.
• Specificity: This monoclonal antibody recognizes the FLAG sequence at the N-terminus
• Immunogen: FLAG peptide sequence DYKDDDDK
• Application: Monoclonal ANTI-FLAG® antibody produced in rabbit has been used in:immunofluorescence[1][2]western blot analysis[3][4]immunocytochemistryindirect ELISALearn more product details in our FLAG® application portal.
• Physical form: Provided as ascites fluid containing 15 mM sodium azide as a preservative.
• Legal Information: ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany