Lysozyme human

Description

• General description: Lysozyme is present in tears, saliva and human milk. The human lysozyme expressed in rice grains is an excellent supplemental source of this antimicrobial protein.[1] Lysozyme is mapped to human chromosome 12q15 and corresponds to a molecular weight of 16.5kDa.[2] It is a positively charged and a C-type lysozyme[3] with α and β structural domains.[4]
• Application: Lysozyme human has been used:in the interaction studies with polyphenon-60 by calorimetric and spectroscopic techniques[5] in the drug release assay of dexamethasone (DEX) - carbon nanotube based delivery system[6] in the surface plasmon resonance (SPR) for measuring its interaction with human angiotensin I-converting enzyme (ACE)[7]
• Biochem/physiol Actions: Lysozyme is an antibacterial enzyme, which acts on the peptidoglycan layer at the on the N-acetylgluosamine (NAG) and N-acetylmuramic acid linkage, resulting in the lysis of microbial cells.[1] It is a potential anti-inflammatory factor and also has anti-fungal and anti-viral functionality.[3] In inflammatory bowel disease, lysozyme is highly expressed in colonic tissue.[2] Point mutations in lysozyme is implicated in the pathogenesis of hereditary non-neuropathic renal amyloidosis.[4]
• Features and Benefits: Lysobac™ is a breakthrough for bacterial cell lysis and can be used in diagnostic applications, bioprocessing and life science research. It is recombinant human lysozyme produced in an animal-free production system. Animal-free production eliminates the safety risk and inconsistent lot-to-lot performance of hen egg white lysozyme. Lysobac™ has significantly higher bioactivity than hen egg white lysozyme. One gram replaces 4 grams of hen egg white lysozyme. It also delivers gentle cell lysis, unlike mechanical lysis which can cause protein shear and reduce protein yields by up to 20%.Advantages:Improves Bacterial Fermentation EfficiencyGreater bacterial lysis activity per mg.1 gram of delivers more lysis activity than 4 grams of HEWLAnimal-free and consistent leading to improved regulatory efficiency for drug manufacturingImproved recombinant protein yield compared to mechanical lysisReduces cost of goods
• Packaging: Package size based on protein content
• Unit Definition: One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).
• Other Notes: View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
• Legal Information: Lysobac is a trademark of InVitria