ABTS™ Buffer

Description

• General description: 2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts as a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.[1]
• Application: ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme.[2][3][4] Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.
• Preparation Note: Working concentration: Flow cytometry: 0.5 μg/100 μl (106 cells); Immunohistocytochemistry: 50 μg/mlWorking concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of ABTS buffer. This solution has a light-green color. In microtiter plates, the solution is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.
• Other Notes: For life science research only. Not for use in diagnostic procedures.
• Legal Information: ABTS is a trademark of Roche