RNase, DNase-free, High Concentration

Description

• General description: A heterogeneous mixture of ribonucleases specially prepared to be free of deoxyribonuclease activity according to the current Quality Control procedures.
• Application: High concentration, DNase-free RNase has been used for:Genomic DNA isolation[1]Isolation of plasmid DNA[1]Degradation of RNA for flow cytometry[2]
• Quality: Absence of DNase activity tested according to the current Quality Control procedures.
• Unit Definition: One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions: A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
• Preparation Note: Working concentration: 50 μg/ml is the recommended working concentration of RNase, DNase-free, for the isolation of genomic DNA.Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0
• Other Notes: For life science research only. Not for use in diagnostic procedures.