216254
BCECF/AM
A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF.
Manufacturer: Sigma Aldrich
Synonym(S): BCECF/AM, 2ʹ,7ʹ- bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester, 2ʹ,7ʹ-bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 MG | 216254-1-MG | In Stock | ₹ 18,200.00 |
216254 - 1 MG
In Stock
Quantity
1
Base Price: ₹ 18,200.00
GST (18%): ₹ 3,276.00
Total Price: ₹ 21,476.00
Quality Level
100
Assay
≥85% (mixture of isomers, HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freezeprotect from light
color
white to yellow
solubility
acetonitrile: 25 mg/mLDMF: solubleDMSO: soluble
fluorescence
λex 500-504 nmλem 525-531 nm
shipped in
ambient
storage temp.
−20°C
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Description
- General description: A variable mixture of cell-permeable ester derivatives of BCECF (Cat. No. 216252) that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules. Typical loading buffer contains 1-10 µM BCECF/AM in serum-free medium that is free of amino acids and buffers containing primary or secondary amines.
- Biochem/physiol Actions: Cell permeable: yes
- Packaging: Packaged under inert gas
- Warning: Toxicity: Irritant (B)
- Reconstitution: Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 week at -20°C.
- Other Notes: Futsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol.256, F957.
- Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Quality Level: 100
Assay: ≥85% (mixture of isomers, HPLC)
form: solid
manufacturer/tradename: Calbiochem®
storage condition: OK to freezeprotect from light
color: white to yellow
solubility: acetonitrile: 25 mg/mLDMF: solubleDMSO: soluble
fluorescence: λex 500-504 nmλem 525-531 nm
shipped in: ambient
storage temp.: −20°C
Quality Level:
100
Assay:
≥85% (mixture of isomers, HPLC)
form:
solid
manufacturer/tradename:
Calbiochem®
storage condition:
OK to freezeprotect from light
color:
white to yellow
solubility:
acetonitrile: 25 mg/mLDMF: solubleDMSO: soluble
fluorescence:
λex 500-504 nmλem 525-531 nm
shipped in:
ambient
storage temp.:
−20°C
Quality Level: 200
Assay: 99%
form: powder
manufacturer/tradename: __
storage condition: __
color: __
solubility: __
fluorescence: __
shipped in: __
storage temp.: __
Quality Level:
200
Assay:
99%
form:
powder
manufacturer/tradename:
__
storage condition:
__
color:
__
solubility:
__
fluorescence:
__
shipped in:
__
storage temp.:
__
Quality Level: 200
Assay: 99%
form: powder
manufacturer/tradename: __
storage condition: __
color: __
solubility: __
fluorescence: __
shipped in: __
storage temp.: __
Quality Level:
200
Assay:
99%
form:
powder
manufacturer/tradename:
__
storage condition:
__
color:
__
solubility:
__
fluorescence:
__
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__
storage temp.:
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Quality Level: 200
Assay: __
form: beads
manufacturer/tradename: __
storage condition: __
color: __
solubility: __
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shipped in: __
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Quality Level:
200
Assay:
__
form:
beads
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