309800

Dihydroethidium

A cell-permeable, chemically-reduced ethidium derivative.

Manufacturer: Sigma Aldrich

CAS Number: 38483-26-0

Synonym(S): Dihydroethidium, 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE

Select a Size

Pack Size SKU Availability Price
25 MG 309800-25-MG In Stock ₹ 24,340.00

309800 - 25 MG

₹ 24,340.00

In Stock

Quantity

1

Base Price: ₹ 24,340.00

GST (18%): ₹ 4,381.20

Total Price: ₹ 28,721.20

Quality Level

100

Assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freezeprotect from light

color

pink

solubility

DMSO: 20 mg/mLDMF: soluble

shipped in

ambient

storage temp.

−20°C

InChI

1S/C21H21N3/c1-2-15-20(13-6-4-3-5-7-13)17-10-14(22)8-9-16(17)18-11-24-12-19(23)21(15)18/h3-12,15,20H,2,22-23H2,1H3

Other Options

Image Product Name Manufacturer Price Range
AI49529
38483-26-0 | HYDROETHIDINE
A2B Chem ₹ 24,470.16 - ₹ 88,297.92

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Description

  • General description: A cell-permeable, chemically reduced ethidium derivative that can be used as a fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, however, following oxidation to ethidium and subsequent intercalation to DNA, a bright red fluorescence is achieved. Often used to determine the level of superoxide anion in cells by flow cytometry.
  • Biochem/physiol Actions: Cell permeable: yes
  • Packaging: Packaged under inert gas
  • Warning: Toxicity: Irritant (B)
  • Reconstitution: Following reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
  • Other Notes: Haugen, T.S., et al. 1999. Eur. Respir. J.14, 1100.Mancini, M., et al. 1997. J. Cell Biol.138, 449.Carter, W.O., et al. 1994. J. Leukoc. Biol.55, 253.Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.Perticarari, S., et al. 1991. Cytometry 12, 687.Bucana, C.D., et al. 1990. Exp. Cell Res.190, 69.Rothe, G., and Valet, G. 1990. J. Leukoc. Biol.47, 440.
  • Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

SAFETY INFORMATION

WGK

WGK 3

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Calbiochem®

storage condition:
OK to freezeprotect from light

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pink

solubility:
DMSO: 20 mg/mLDMF: soluble

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ambient

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−20°C

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1S/C21H21N3/c1-2-15-20(13-6-4-3-5-7-13)17-10-14(22)8-9-16(17)18-11-24-12-19(23)21(15)18/h3-12,15,20H,2,22-23H2,1H3

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