382061

Bisbenzimide H 33258 Fluorochrome, Trihydrochloride

Membrane-permeable, adenine-thymine-specific fluorescent stain.

Manufacturer: Sigma Aldrich

CAS Number: 23491-45-4

Synonym(S): Bisbenzimide H 33258 Fluorochrome, Trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)-benzimidazole, 3HCl, HOE 33258

Select a Size

Pack Size SKU Availability Price
100 MG 382061-100-MG In Stock ₹ 14,880.00
250 MG 382061-250-MG In Stock ₹ 21,020.00
1 G 382061-1-G In Stock ₹ 61,890.01

382061 - 100 MG

₹ 14,880.00

In Stock

Quantity

1

Base Price: ₹ 14,880.00

GST (18%): ₹ 2,678.40

Total Price: ₹ 17,558.40

Quality Level

100

Assay

≥98% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freezeprotect from light

color

yellow-green

fluorescence

λex 346 nmλem 460 nm

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,26,28H,10-13H2,1H3,(H,27,29);3*1H

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Description

  • General description: Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.
  • Biochem/physiol Actions: Cell permeable: yes
  • Warning: Toxicity: Carcinogenic / Teratogenic (D)
  • Preparation Note: Precipitates in phosphate buffered solutions
  • Reconstitution: Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 weeks at 4°C.
  • Other Notes: Werstuck, G., and Green, M.R. 1998. Science 282, 296.Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.Tarshis, M., et al. 1994. J. Immunol. Methods168, 245.Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.Oberhammer, F., et al. 1993. EMBO J. 12, 3679.Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods162, 41.Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA85, 8939.Cesarone, C.F., et al. 1979. Anal. Biochem.100, 188.Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem.24, 24.
  • Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,26,28H,10-13H2,1H3,(H,27,29);3*1H

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InChI:
1S/C18H16N2O8S2.2Na/c1-10-7-14(16(28-2)9-17(10)30(25,26)27)19-20-18-13-5-4-12(29(22,23)24)8-11(13)3-6-15(18)21;;/h3-9,21H,1-2H3,(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2/b20-19+;;