Description

• Application: Clostripain from Clostridium histolyticum has been used as a proteolytic enzyme in perfusate to detect its effect on tube hematocrit.[1] It has also been used in limited proteolysis of DNA polymerase (gp43) of phage T4 (RB69 gp43).[2]
• Biochem/physiol Actions: Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds.[3]
• Packaging: Bottomless glass bottle. Contents are inside inserted fused cone.
• Quality: Purified, essentially salt-free
• Unit Definition: One unit will hydrolyze 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of 2.5 mM DTT.
• Analysis Note: The enzyme must be activated for 2-3 hours before use by dissolving in 2.5 mM dithiothreitol containing 1.0 mM calcium acetate.