Description

• General description: Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread.
• Application: Neuraminidase from Clostridium perfringens has been used in a study to assess binding with human T lymphocytes in sheep pretreated with neuraminidase. [1] It has also been used in a study to investigate the effect of bile salts on the action of hydrolysis by neuraminidase. [2]
• Biochem/physiol Actions: Neuraminidase can increase aggregation in certain cell lines by removing exposed negatively charged sialic acid residues on the cell surface. [3]
• Unit Definition: One unit will liberate 1.0 micromole of N-acetyl neuraminic acid per minute at pH 5.0 at 37 °C using bovine submaxillary mucin.One unit will hydrolyze 1.0 micromole of 2′-(4-methylumbelliferyl)-a-D-N-actetylneuraminic acid per minute at pH 5.0 at 37 °C (using 4MU-NANA as a substrate)
• Preparation Note: Prepared by salt fractionation.
• Analysis Note: Package sizes based on 4MU-NANA units