Description

• General description: Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.
• Application: Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. [1] It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase. [2]
• Biochem/physiol Actions: Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells. [1]
• Quality: Preservative free.
• Unit Definition: One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.
• Physical form: Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl2.
• Preparation Note: Chromatographically purified.