N3254
4-Nitrophenyl phosphate di(tris) salt
phosphatase substrate, ≥97% (enzymatic), powder
Manufacturer: Sigma Aldrich
CAS Number: 68189-42-4
Synonym(S): 4-Nitrophenyl phosphate bis(tris) salt
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 G | N3254-1-G | In Stock | ₹ 4,178.45 |
| 5 G | N3254-5-G | In Stock | ₹ 10,608.50 |
| 25 G | N3254-25-G | In Stock | ₹ 39,933.43 |
| 100 G | N3254-100-G | In Stock | ₹ 1,08,022.68 |
N3254 - 1 G
In Stock
Quantity
1
Base Price: ₹ 4,178.45
GST (18%): ₹ 752.121
Total Price: ₹ 4,930.571
product name
4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate
Quality Level
100
Assay
≥97% (enzymatic)
form
powder
solubility
water: 50 mg/mL, clear, colorless to light yellow
storage temp.
−20°C
SMILES string
NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI
1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
InChI key
XXAXKCWOTRABOW-UHFFFAOYSA-N
Other Options
| Image | Product Name | Manufacturer | Price Range | |
|---|---|---|---|---|
 | Sigma Aldrich Fine Chemicals Biosciences 4-Nitrophenyl phosphate di(tris) salt phosphatase substrate | 68189-42-4 | MFCD00037009 | 100g | Sigma Aldrich Fine Chemicals Biosciences | ₹ 1,07,120.40 | |
 | Chem-Impex International, Inc. 4-Nitrophenyl phosphate di(tris) salt | 68189-42-4 | MFCD00037009 | 100G | Chem-Impex International, Inc. | ₹ 18,156.00 | |
| | Chem-Impex International, Inc. 4-Nitrophenyl phosphate di(tris) salt | 68189-42-4 | MFCD00037009 | 5G | Chem-Impex International, Inc. | ₹ 3,493.25 | |
| | Sigma Aldrich Fine Chemicals Biosciences 4-Nitrophenyl phosphate di(tris) salt phosphatase substrate | 68189-42-4 | MFCD00037009 | 5G | Sigma Aldrich Fine Chemicals Biosciences | ₹ 10,825.96 | |
 | 4-Nitrophenyl phosphate ditromethamine | ChemScene | ₹ 7,921.00 | |
 | 68189-42-4 | 4-Nitrophenyl phosphate di(tris) salt | A2B Chem | ₹ 2,136.00 - ₹ 1,16,857.00 |
Related Products
Description
- Application: 4-Nitrophenyl phosphate di(tris) salt has been used:as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates[1]to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)[2]for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)[2]
- Biochem/physiol Actions: p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.[3]
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Compare Similar Items
Show Difference
product name: 4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate
Quality Level: 100
Assay: ≥97% (enzymatic)
form: powder
solubility: water: 50 mg/mL, clear, colorless to light yellow
storage temp.: −20°C
SMILES string: NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI: 1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
InChI key: XXAXKCWOTRABOW-UHFFFAOYSA-N
product name:
4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate
Quality Level:
100
Assay:
≥97% (enzymatic)
form:
powder
solubility:
water: 50 mg/mL, clear, colorless to light yellow
storage temp.:
−20°C
SMILES string:
NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI:
1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
InChI key:
XXAXKCWOTRABOW-UHFFFAOYSA-N
product name: __
Quality Level: 100
Assay: 98%
form: crystals
solubility: __
storage temp.: __
SMILES string: O=C1C[C@@H]2CC[C@H]1C2
InChI: 1S/C7H10O/c8-7-4-5-1-2-6(7)3-5/h5-6H,1-4H2/t5-,6+/m1/s1
InChI key: KPMKEVXVVHNIEY-RITPCOANSA-N
product name:
__
Quality Level:
100
Assay:
98%
form:
crystals
solubility:
__
storage temp.:
__
SMILES string:
O=C1C[C@@H]2CC[C@H]1C2
InChI:
1S/C7H10O/c8-7-4-5-1-2-6(7)3-5/h5-6H,1-4H2/t5-,6+/m1/s1
InChI key:
KPMKEVXVVHNIEY-RITPCOANSA-N
product name: __
Quality Level: __
Assay: __
form: powder
solubility: __
storage temp.: 2-8°C
SMILES string: __
InChI: __
InChI key: __
product name:
__
Quality Level:
__
Assay:
__
form:
powder
solubility:
__
storage temp.:
2-8°C
SMILES string:
__
InChI:
__
InChI key:
__
product name: __
Quality Level: 200
Assay: __
form: solid
solubility: __
storage temp.: 2-8°C
SMILES string: O.[Na+].CCOc1ccc2ccccc2c1C(=O)N[C@H]3C4SC(C)(C)[C@@H](N4C3=O)C([O-])=O
InChI: 1S/C21H22N2O5S.Na.H2O/c1-4-28-13-10-9-11-7-5-6-8-12(11)14(13)17(24)22-15-18(25)23-16(20(26)27)21(2,3)29-19(15)23;;/h5-10,15-16,19H,4H2,1-3H3,(H,22,24)(H,26,27);;1H2/q;+1;/p-1/t15-,16+,19-;;/m1../s1
InChI key: OCXSDHJRMYFTMA-KMFBOIRUSA-M
product name:
__
Quality Level:
200
Assay:
__
form:
solid
solubility:
__
storage temp.:
2-8°C
SMILES string:
O.[Na+].CCOc1ccc2ccccc2c1C(=O)N[C@H]3C4SC(C)(C)[C@@H](N4C3=O)C([O-])=O
InChI:
1S/C21H22N2O5S.Na.H2O/c1-4-28-13-10-9-11-7-5-6-8-12(11)14(13)17(24)22-15-18(25)23-16(20(26)27)21(2,3)29-19(15)23;;/h5-10,15-16,19H,4H2,1-3H3,(H,22,24)(H,26,27);;1H2/q;+1;/p-1/t15-,16+,19-;;/m1../s1
InChI key:
OCXSDHJRMYFTMA-KMFBOIRUSA-M