Peroxidase, Maleimide Activated from horseradish

Description

• General description: Peroxidase (POD) is an enzyme that includes a group of specific enzymes such as nicotinamide adenine dinucleotide (NAD) peroxidase, NADP peroxidase, fatty acid peroxidase and a group of non-specific enzymes from various sources. It is present in animals, higher plants and other organisms.[1]
• Application: For direct conjugation to compounds containing free sulfhydryl groups for use as an enzyme-linked detection reagent.[2]Instructions for use included. In a study on horseradish peroxidase, the Maleimide compounds succinimidyl 4-maleimidobutyrate and succinimidyl 6-maleimidohexanoate have been shown to be the most effective in the conjugation of Fab′ to horseradish peroxidase. [3] This enzyme has also been used in a study to investigate the effectiveness in staining a mitochondrial protein, lipoamide dehydrogenase. [4]
• Biochem/physiol Actions: Peroxidase (POD) helps in the dehydrogenation of several organic compounds like phenols, aromatic amines, hydroquinones etc.[1]
• Other Notes: Type VI peroxidase activated with maleimidocaproic acid N-hydroxysuccinimide ester.
• Unit Definition: One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 seconds at pH 6.0 at 20°C.
• Physical form: Lyophilized powder containing trehalose and sodium citrate