PNGase F from Elizabethkingia meningoseptica
recombinant, expressed in E. coli, set of 100 units nanomolar unit
Manufacturer: Sigma Aldrich
CAS Number: 83534-39-8
Synonym(S): N-Glycanase®, N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 SET | P9120-1-SET | In Stock | ₹ 78,510.30 |
P9120 - 1 SET
In Stock
Quantity
1
Base Price: ₹ 78,510.30
GST (18%): ₹ 14,131.854
Total Price: ₹ 92,642.154
recombinant
expressed in E. coli
Quality Level
200
conjugate
(N-linked)
specific activity
≥10 units/mg protein
mol wt
36 kDa
packaging
set of 100 units nanomolar unit
shipped in
wet ice
storage temp.
2-8°C
Other Options
| Image | Product Name | Manufacturer | Price Range | |
|---|---|---|---|---|
 | PNGase F from Elizabethkingia meningoseptica | Sigma Aldrich | ₹ 37,518.00 - ₹ 1,82,739.30 | |
| | PNGase F from Elizabethkingia miricola | Sigma Aldrich | ₹ 34,720.80 - ₹ 59,584.80 | |
 | N-Glycosidase F, Elizabethkingia meningosepticum | Sigma Aldrich | ₹ 42,350.00 | |
 | Glycopeptidase A from almonds | Sigma Aldrich | ₹ 1,06,038.30 | |
 | N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli | Sigma Aldrich | ₹ 59,040.00 | |
| | PNGase F from Elizabethkingia meningoseptica | Sigma Aldrich | ₹ 28,726.80 - ₹ 1,43,278.80 | |
| | PNGase F from Elizabethkingia meningoseptica | Sigma Aldrich | ₹ 28,427.10 - ₹ 1,39,427.10 | |
 | 83534-39-8 | PNGase F | A2B Chem | -- |
Related Products
Description
- Application: PNGase F from Elizabethkingia meningoseptica has been used in deglycosylationof recombinant soybean agglutinin (rSBA) in Nicotiana benthamiana (NbrSBA) and Solanum tuberosum (StrSBA)[1]of frontal cortical lysate to verify the glycosylation profile of β-secretase (BACE proteins)[2]of cell lysate for evaluating the siRNA silencing of cellular prion protein (PrPc) post transfection[3]
- Biochem/physiol Actions: PNGase F cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. It deaminates the asparagine to aspartic acid, but leaves the oligosaccharide intact. PNGase F will not remove oligosaccharides containing α(1-3)-linked core fucose, commonly found in plant glycoproteins. A tripeptide with the oligosaccharide-linked asparagine as the central residue is the minimal substrate for PNGase F[4].
- Packaging: Each set includes enzyme, two formulations of 5× reaction buffer (for routine and mass spectrometry downstream analysis), detergent and denaturation solutions
- Unit Definition: One unit will catalyze the release of N-linked oligosaccharides from 1 micromole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.
- Legal Information: N-Glycanase is a registered trademark of Agilent Technologies Inc
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
P273 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338 - P308 + P313
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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recombinant: expressed in E. coli
Quality Level: 200
conjugate: (N-linked)
specific activity: ≥10 units/mg protein
mol wt: 36 kDa
packaging: set of 100 units nanomolar unit
shipped in: wet ice
storage temp.: 2-8°C
recombinant: __
Quality Level: 200
conjugate: __
specific activity: __
mol wt: __
packaging: __
shipped in: ambient
storage temp.: room temp
recombinant: __
Quality Level: 200
conjugate: __
specific activity: __
mol wt: __
packaging: __
shipped in: __
storage temp.: −20°C