Description

• General description: Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.
• Warning: Toxicity: Standard Handling (A)
• Unit Definition: One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.
• Physical form: In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
• Other Notes: Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany