Invitrogen™Silencer™ Select Negative Control No. 2 siRNA

Description

• Ambion™ Silencer ™ SelectNegative Control #2 siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment
• Premium-quality siRNA, highly purified and ready to use Functionally proven to have minimal effects on cell proliferation and viability Include Silencer ™ Select modifications for enhanced specificity For use in human, mouse, and rat cells What are Silencer ™ Select siRNAs? Silencer ™ Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications, to yield siRNAs with unrivalled efficacy, potency, and specificity
• The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data
• Silencer ™ Select Negative Control #2 siRNA: Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples
• Silencer ™ Select Negative Control #2 siRNA has been carefully designed and extensively tested, and has no significant sequence similarity to mouse, rat, or human gene sequences
• This negative control siRNA has been tested by microarray analysis and shown to have minimal effects on gene expression
• It has also been tested in multi-parametric, cell-based assays and shown to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested
• Quality Control: Silencer ™ Select siRNAs are manufactured in a state-of-the-art facility under the highest quality standards
• Rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity
• Each siRNA is also assessed by gel electrophoresis to confirm that the strands anneal properly
• Accessory Product: siPORT™ NeoFX ™ Transfection Agent (SKU #AM4510 and #AM4511) siPORT™ NeoFX ™ Transfection Agent is a versatile, lipid-based transfection agent for the efficient transfection of siRNA into cultured cells
• The protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications
• Silencer ™ Select siRNA controls have specific chemical modifications; they are designed to be used with Silencer ™ Select siRNAs
• If you are using other siRNA products, such as BLOCK-iT™ siRNAs, Ambion™ Silencer ™ siRNAs, or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.