4532027

Thermo Scientific™ Pierce™ Recombinant Protein L

Detect mouse and human antibodies containing kappa light chains in immunoassays using this purified, biotinylated or peroxidase-conjugated, recombinant Protein L.

Manufacturer: Fischer Scientific

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Product Line

Pierce™

Protein Subtype

Protein L

Content And Storage

Upon receipt store at 4°C.Note: Store reconstituted product for up to one month at 4°C. For long-term storage, aliquot, add an equal volume of glycerol and store at -20°C.

Form

Powder

Target

Antibodies

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Description

  • Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein L enable probing and detection of mouse and human antibodies containing kappa light chains in Western blotting, ELISA, IHC and other immunoassay protocols
  • Protein L is an immunoglobulin-binding protein that was originally derived from the bacteria Peptostreptococcus magnus, but is now produced recombinantly
  • Protein L has the unique ability to bind through kappa light chain interactions without interfering with an antibody's antigen-binding site
  • This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins
  • Protein L will bind to all classes of Ig (IgG, IgM, IgA, IgE and IgD)
  • Protein L will also bind single chain variable fragments (scFv) and Fab fragments
  • Protein L binds kappa I, III, and IV in human and kappa I on mouse
  • Highlights: Contains four immunoglobulin-binding domains per protein Binds members of all classes of Ig (IgG, IgM, IgA, IgE and IgD) that contain kappa light chains Binds single chain variable fragments (scFv) and Fab fragments that contain kappa light chains

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Product Line:
Pierce™

Protein Subtype:
Protein L

Content And Storage:
Upon receipt store at 4°C.Note: Store reconstituted product for up to one month at 4°C. For long-term storage, aliquot, add an equal volume of glycerol and store at -20°C.

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Powder

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